| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | - |
| Strain: | CLIP2.041138 |
| Chromosome: | chromosome 3 |
| Location: | 5737987 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g187250 | (1 of 1) PF05514 - HR-like lesion-inducing (HR_lesion) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGGGAACTGGGCTGGCAGCTCGACGCCCAGTTGCCGCAGCGCCGTTACC |
| Internal bar code: | GTTCGGTGTCTTGAGTTCTGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 851 |
| LEAP-Seq percent confirming: | 70.0 |
| LEAP-Seq n confirming: | 7 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGAGCGCTATTTGGACAGCA |
| Suggested primer 2: | GCAAGCGTCAGTATGCCATG |