| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.041154 |
| Chromosome: | chromosome 3 |
| Location: | 2479403 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g159200 | UBC8 | (1 of 2) K10589 - ubiquitin-protein ligase E3 C (UBE3C); Putative ubiquitin-protein ligase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGCTGCTGGCTGCTGTATCCAGTATACATAGCTGGCAGTAACGCGAAGC |
| Internal bar code: | CGTTGCGTGGCCTTTTTATGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2310 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 47 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 47 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATCTGGGCCACACAAGATGG |
| Suggested primer 2: | CACATGGGACTATGGGAGGC |