| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.041198 |
| Chromosome: | chromosome 12 |
| Location: | 9453076 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g540900 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AACAGTGACATGGGTCAGCACGCGCGGCAGGCACTAGATATGCATTTCAT |
| Internal bar code: | AGCACTAAAGGACGACATCGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3240 |
| LEAP-Seq percent confirming: | 91.4286 |
| LEAP-Seq n confirming: | 64 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 70 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGAAGTTGTTCCGGTCGGA |
| Suggested primer 2: | AAGTTGCTCTGCTGCGTTTG |