Insertion junction: CLIP2.041201_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.041201
Chromosome:chromosome 17
Location:3482552
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre17.g723950 FAP397 Flagellar Associated Protein 397 with UDP-galactose 4-epimerase domain intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGAGTAAAAGCCCGACGTGTTCGGACCCCAGGGGCCTGGATGGACCCAGC
Internal bar code:ATGCCATGCTGTCCATGATTGG

Confirmation - LEAP-Seq

LEAP-Seq distance:3445
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:38
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:38

Suggested primers for confirmation by PCR

Suggested primer 1:GTAGGTGGCTGGGAGGACTA
Suggested primer 2:CCTCACCCACGATGAACACA