| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.041204 |
| Chromosome: | chromosome 3 |
| Location: | 5988944 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g189450 | (1 of 2) PTHR22769//PTHR22769:SF33 - MUTT/NUDIX HYDROLASE // NUCLEOSIDE DIPHOSPHATE-LINKED MOIETY X MOTIF 17 | CDS/intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCGCGGGTGCGGCAGGCGCGCGGGAGCAGCGGCAACCGGCCTGGGGCTG |
| Internal bar code: | TATGGTCAGCTTTTACTGTTCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5302 |
| LEAP-Seq percent confirming: | 93.617 |
| LEAP-Seq n confirming: | 88 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 94 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCGGAGCCGGATATTGCATC |
| Suggested primer 2: | ATCAACACCCTGCATGTCGT |