| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.041214 |
| Chromosome: | chromosome 3 |
| Location: | 8763821 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g207713 | ISA3 | Isoamylase, starch debranching enzyme 3; (1 of 2) 3.2.1.68 - Isoamylase / Debranching enzyme | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CATGGTGTCCTACAACGACAAACACAACGACGCCAACGGTGCGTGCATCG |
| Internal bar code: | GATCGCCACGGAGATTCAATTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 296 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 4 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GATGATCTCATCGCCTGGCA |
| Suggested primer 2: | GGTCGGTTTGCAAAGCATGT |