| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.041228 |
| Chromosome: | chromosome 6 |
| Location: | 7517576 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g301100 | (1 of 5) PF04232 - Stage V sporulation protein S (SpoVS) (SpoVS) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCTCTACACAGGTGACGGCGCACAGCCGCCCCAACGTGGTGTCCAACAT |
| Internal bar code: | TTTCCCTGTTGTCCCCTGGAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3955 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 55 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 55 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTAGACCACCCACCGACCTA |
| Suggested primer 2: | ACCTACACAACTTCCTGGCG |