| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.041230 |
| Chromosome: | chromosome 12 |
| Location: | 4087271 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g516750 | (1 of 55) PTHR23257//PTHR23257:SF394 - SERINE-THREONINE PROTEIN KINASE // SUBFAMILY NOT NAMED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCGCCTGCGGGAGCAAGAAGAAGTGCGAGCCGTGGCAAAACAAATATGT |
| Internal bar code: | ATATCGTGTGAGGGGAGGTTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3022 |
| LEAP-Seq percent confirming: | 97.5 |
| LEAP-Seq n confirming: | 39 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 40 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACACGCCTCACTCACTCTT |
| Suggested primer 2: | CCTAATCCTCTCCAGCGCTG |