| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.041232 |
| Chromosome: | chromosome 2 |
| Location: | 6553569 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g389200 | UMM8 | UbiE/COQ5 methyltransferase family protein; (1 of 1) 2.1.1.288 - Aklanonic acid methyltransferase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GACCTCGCCACAGGTGAGCACGGCGCGCTCGGAACGGGTAAGGAGTCCAC |
| Internal bar code: | AGTATGTGAATGACAAACAACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4004 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 75 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 75 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTTAGCGCTTCCAAATCCG |
| Suggested primer 2: | ACGCTGGACACAATGCCTAA |