| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.041236 |
| Chromosome: | chromosome 14 |
| Location: | 3758675 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g631850 | (1 of 1) 3.1.3.74 - Pyridoxal phosphatase / Vitamin B6-phosphate phosphatase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTTGCATGAGCAGCACACGCCAATTTGCATGGTTCCACTTCGCCAACCC |
| Internal bar code: | CTGTTCACGGTACGCATCAAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3069 |
| LEAP-Seq percent confirming: | 87.7551 |
| LEAP-Seq n confirming: | 43 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 49 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACAGCTCACAGCAACTCCTC |
| Suggested primer 2: | AGCTCATTCCCTCAACAGCC |