| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.041238 |
| Chromosome: | chromosome 5 |
| Location: | 2278448 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g238100 | PTK25 | (1 of 66) 2.7.12.1 - Dual-specificity kinase; Protein tyrosine kinase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGACTCTGAGACGGGCGGCTACGTGATGGCCAAGGTCAACTCCAGCAAA |
| Internal bar code: | TTTCCAAAGCGACTCGGGGCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1424 |
| LEAP-Seq percent confirming: | 75.0 |
| LEAP-Seq n confirming: | 15 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTTGCCTTGGGTTTGACGG |
| Suggested primer 2: | ACGGGACTGTGGTATAGCCT |