Insertion junction: CLIP2.041239_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.041239
Chromosome:chromosome 10
Location:2757832
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre10.g438200 (1 of 2) IPR001841//IPR001876//IPR016024 - Zinc finger, RING-type // Zinc finger, RanBP2-type // Armadillo-type fold intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCGAACCCACATCACACAACACGCCAGACGAGGACACGCCCCGCCGAATC
Internal bar code:CGTGTTTCTGACTAGTAGTCGC

Confirmation - LEAP-Seq

LEAP-Seq distance:2959
LEAP-Seq percent confirming:95.4545
LEAP-Seq n confirming:42
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:44

Suggested primers for confirmation by PCR

Suggested primer 1:ACACTTAGACACAGGACGCG
Suggested primer 2:TTCAATGGCAGGCAGGGTAG