Insertion junction: CLIP2.041264_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.041264
Chromosome:chromosome 2
Location:2739622
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g800206 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTGACGACGGTTGGGCCCGGCCGCGTGATTTGCGCAAGGACCACATTGGC
Internal bar code:TCGTGGAAGAGTCGGTCTGGCG

Confirmation - LEAP-Seq

LEAP-Seq distance:4689
LEAP-Seq percent confirming:76.7742
LEAP-Seq n confirming:119
LEAP-Seq n nonconfirming:36
LEAP-Seq n unique pos:155

Suggested primers for confirmation by PCR

Suggested primer 1:GGACGATCGCTCTAGTGTGG
Suggested primer 2:GTGGCTGTGGTACTCGTTCA