| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.041286 |
| Chromosome: | chromosome 6 |
| Location: | 5485725 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g286300 | UMM5,COQ5B | (1 of 2) PTHR10108//PTHR10108:SF830 - METHYLTRANSFERASE // SUBFAMILY NOT NAMED; Ubiquinone/menaquinone biosynthesis methyltransferase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGCACACAACCTGACGAGGCAGGAGCGGGGGGGGGGGGGTCGGAGGGTG |
| Internal bar code: | TCAAAGAAATTTGTATACATTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3960 |
| LEAP-Seq percent confirming: | 98.7179 |
| LEAP-Seq n confirming: | 77 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 78 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCAATCAGGTCCATGCATG |
| Suggested primer 2: | AGCACAGGTACTTGGTGTCG |