| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.041300 |
| Chromosome: | chromosome 17 |
| Location: | 1645507 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g707750 | (1 of 1) PTHR31040//PTHR31040:SF1 - FAMILY NOT NAMED // NURIM | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCCTGCACACCCAGAAATGTCGTACAGTCCGTATTGTTGACTAAATCGT |
| Internal bar code: | AAGCAGGTGCGATTCTCAGTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1557 |
| LEAP-Seq percent confirming: | 82.3529 |
| LEAP-Seq n confirming: | 42 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 51 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTACACGTTGAGCACCAGG |
| Suggested primer 2: | ACACCTCTTGATGGTCTGCG |