Insertion junction: CLIP2.041314_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.041314
Chromosome:chromosome 10
Location:2738843
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre10.g438000 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATACCTGTGGGCCGTCTCCGCCTTCCACTTCCGCGAAGGCGCCCACATGT
Internal bar code:AACCAGACGTTGTAACTTGCAA

Confirmation - LEAP-Seq

LEAP-Seq distance:5109
LEAP-Seq percent confirming:87.5
LEAP-Seq n confirming:42
LEAP-Seq n nonconfirming:6
LEAP-Seq n unique pos:48

Suggested primers for confirmation by PCR

Suggested primer 1:GGCCATATCCTCATGCACGA
Suggested primer 2:CCAGCGGAAGAACCAACTCT