| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.041327 |
| Chromosome: | chromosome 10 |
| Location: | 5886272 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g460700 | (1 of 15) PTHR12291:SF23 - PROTEIN MEC-15 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCAACGGCACCACCATCAAAGCCGCACGGAAGACCCTGCTGCCTCAGAA |
| Internal bar code: | GGATATGCATTGAATGGCCGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3921 |
| LEAP-Seq percent confirming: | 84.8101 |
| LEAP-Seq n confirming: | 67 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 79 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAACAAACCAAGGCAGCCTG |
| Suggested primer 2: | CCCTTTCCATGTCTGCCCAT |