Insertion junction: CLIP2.041328_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.041328
Chromosome:chromosome 4
Location:2874848
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre04.g224400 (1 of 1) 3.6.3.25//3.6.3.44 - Sulfate-transporting ATPase // Xenobiotic-transporting ATPase / Steroid-transporting ATPase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CACCCATGGTGCGTACGGCACACGTGTCTTCTCGCGGGCAGCAGTCTCAG
Internal bar code:AGTCGATGCGTCTGTGGGATCG

Confirmation - LEAP-Seq

LEAP-Seq distance:5824
LEAP-Seq percent confirming:94.7826
LEAP-Seq n confirming:109
LEAP-Seq n nonconfirming:6
LEAP-Seq n unique pos:115

Suggested primers for confirmation by PCR

Suggested primer 1:CTGTGGCGGTATGGGTATCC
Suggested primer 2:GCGGGATGGAAGGAAAGGAA