Insertion junction: CLIP2.041337_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.041337
Chromosome:chromosome 9
Location:3473798
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre09.g396102 (1 of 1) K08856 - serine/threonine kinase 16 [EC:2.7.11.1] (STK16) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CAACACACGCGCAACACACGCACGTGTAAAACACACACGCTCACCGTGCC
Internal bar code:GTGTGTGAATGCGTGGCCCAGG

Confirmation - LEAP-Seq

LEAP-Seq distance:50
LEAP-Seq percent confirming:8.04598
LEAP-Seq n confirming:7
LEAP-Seq n nonconfirming:80
LEAP-Seq n unique pos:87

Suggested primers for confirmation by PCR

Suggested primer 1:TAGTCCAGCAGGGGCAGTAT
Suggested primer 2:TTTCAGGTCGCAAAACGCAG