Insertion junction: CLIP2.041340_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.041340
Chromosome:chromosome 12
Location:4228566
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g518100 FAL16 Chlamydomonas-specific family protein; (1 of 44) IPR000595 - Cyclic nucleotide-binding domain CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTCAAGAGAAAGGGACAGCGTGCGCAGCTGGGTGAGCTGCACGAGGCTGC
Internal bar code:TGCGTATATCGTACACTGTCAG

Confirmation - LEAP-Seq

LEAP-Seq distance:3516
LEAP-Seq percent confirming:98.7805
LEAP-Seq n confirming:81
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:82

Suggested primers for confirmation by PCR

Suggested primer 1:GCACGACCCTACAGTTTCCA
Suggested primer 2:CAGTAACAGGGGCCTCCAAG