Insertion junction: CLIP2.041354_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.041354
Chromosome:chromosome 8
Location:3727364
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre08.g380550 SDR14 (1 of 2) IPR002347//IPR013968 - Glucose/ribitol dehydrogenase // Polyketide synthase, ketoreductase domain; Short-chain dehydrogenase/reductase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCCGCCACCGCTCCGCCACCGCCACCGCCACCCCGCCACCGCCACCCCG
Internal bar code:ATTCGGAGAGAAGCAAGTATCG

Confirmation - LEAP-Seq

LEAP-Seq distance:43
LEAP-Seq percent confirming:9.09091
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:10
LEAP-Seq n unique pos:11

Suggested primers for confirmation by PCR

Suggested primer 1:CGCTAAGCTGACGTTGATGC
Suggested primer 2:CCCTGCCGTCCTTGATAGAC