Insertion cassette: | CIB2 |
Side of cassette: | 5' |
Strand: | + |
Strain: | CLIP2.041356 |
Chromosome: | chromosome 11 |
Location: | 4066706 |
Confidence (%): | 80 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre11.g480851 | RRA3 | (1 of 3) PTHR10994//PTHR10994:SF3 - RETICULON // ARABINOSYLTRANSFERASE; Reduced residual arabinose 3 | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAAGAGCACAAGGAAAGCGCAAAGACAGTGTATGTAGCGATGCAGCAACG |
Internal bar code: | TGCGCGGTGGCCTGAAGCGGTT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 181 |
LEAP-Seq percent confirming: | 43.3962 |
LEAP-Seq n confirming: | 23 |
LEAP-Seq n nonconfirming: | 30 |
LEAP-Seq n unique pos: | 53 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TGCGATGGTCGATTAGGAGC |
Suggested primer 2: | ATACCGATGACCCGATGCAC |