| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.041369 |
| Chromosome: | chromosome 9 |
| Location: | 5908678 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g411975 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCAATGTAAGGAGCCGCAGCGTTGGAAACCCAACGACGGCTGCAGGCGC |
| Internal bar code: | GTTTTTGCTCTTGCCTAAGCTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2462 |
| LEAP-Seq percent confirming: | 33.871 |
| LEAP-Seq n confirming: | 21 |
| LEAP-Seq n nonconfirming: | 41 |
| LEAP-Seq n unique pos: | 62 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTCTGTCCTCTTGGAGCTC |
| Suggested primer 2: | ACGGCTCGTTTCATTTTGCC |