Insertion junction: CLIP2.041385_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.041385
Chromosome:chromosome 16
Location:2888404
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g663200 FAP295 (1 of 2) PTHR24353//PTHR24353:SF37 - CYCLIC NUCLEOTIDE-DEPENDENT PROTEIN KINASE // PROTEIN KINASE DC1-RELATED; Flagellar Associated Protein 295 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTAGGTCTGCAAACCCTTGCTGCTTTCATCTTAACGCACCTCGAACTTCC
Internal bar code:TCGAAACACCTGCTGTTCCATG

Confirmation - LEAP-Seq

LEAP-Seq distance:756
LEAP-Seq percent confirming:20.0
LEAP-Seq n confirming:3
LEAP-Seq n nonconfirming:12
LEAP-Seq n unique pos:15

Suggested primers for confirmation by PCR

Suggested primer 1:TCAAGTCGCAACCAGAGGAC
Suggested primer 2:CTGGCGTGTTCTGGTACACT