Insertion junction: CLIP2.041403_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.041403
Chromosome:chromosome 11
Location:921296
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre11.g467656 (1 of 9) IPR007112//IPR009009 - Expansin/pollen allergen, DPBB domain // RlpA-like double-psi beta-barrel domain intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTGCTCGCAGCTGCGGCTACACTAACGCGGACGGCTCCATGCCCTTCCCG
Internal bar code:TGGCTCCTAGGCTGACTGTGGC

Confirmation - LEAP-Seq

LEAP-Seq distance:951
LEAP-Seq percent confirming:70.0
LEAP-Seq n confirming:28
LEAP-Seq n nonconfirming:12
LEAP-Seq n unique pos:40

Suggested primers for confirmation by PCR

Suggested primer 1:CCACCACATCACACATCCCA
Suggested primer 2:GGTTGTGGATACCAGCCCAA