Insertion junction: CLIP2.041412_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.041412
Chromosome:chromosome 6
Location:8207875
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g306200 CDS
Cre06.g306250 Hypothetical protein; (1 of 9) 2.7.10.1 - Receptor protein-tyrosine kinase / Receptor protein tyrosine kinase 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACATGGTGGCGTCGATGGTGTACCACCCGCACGACACATATTTGCCGCCG
Internal bar code:ACTCTGCTCACGGGGCAAAGTT

Confirmation - LEAP-Seq

LEAP-Seq distance:3001
LEAP-Seq percent confirming:61.1111
LEAP-Seq n confirming:11
LEAP-Seq n nonconfirming:7
LEAP-Seq n unique pos:18

Suggested primers for confirmation by PCR

Suggested primer 1:CGTCTGCAACAATAGCTCGC
Suggested primer 2:CGGGGAGAGGGGTAAGAGAA