Insertion cassette: | CIB2 |
Side of cassette: | 3' truncated? |
Strand: | + |
Strain: | CLIP2.041415 |
Chromosome: | chromosome 2 |
Location: | 5781741 |
Confidence (%): | 80 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre02.g116950 | HDA1 | RPD3/HDA1 type histone deacetylase; (1 of 2) 3.5.1.4 - Amidase / Acylase | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGAAGACGAGGGGCCGGGGACCATCGTGAGCGCTCCTGTGCCACCAGCG |
Internal bar code: | ACAATTAGGGTGGTAATTTTCG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 2051 |
LEAP-Seq percent confirming: | 93.3333 |
LEAP-Seq n confirming: | 42 |
LEAP-Seq n nonconfirming: | 3 |
LEAP-Seq n unique pos: | 45 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TTGGGCTGGAACTTACGACC |
Suggested primer 2: | AAATGTACGGCCAGGTCAGG |