| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.041422 |
| Chromosome: | chromosome 2 |
| Location: | 8153564 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g144005 | (1 of 4) 2.3.1.43 - Phosphatidylcholine--sterol O-acyltransferase / Phospholipid--cholesterol acyltransferase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTACTACAAGGGCAGGTAGGAGGGACGGCGGGCAGCGGCGGGCTTCCTCT |
| Internal bar code: | TGAGTTCCACTTTGGTTAAATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 490 |
| LEAP-Seq percent confirming: | 64.5833 |
| LEAP-Seq n confirming: | 31 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 48 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGAGGTGGGAGCGATTAGGT |
| Suggested primer 2: | TACATCACGTATGGCTCGGC |