| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.041435 |
| Chromosome: | chromosome 3 |
| Location: | 1886278 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g154750 | (1 of 4) 2.3.1.43 - Phosphatidylcholine--sterol O-acyltransferase / Phospholipid--cholesterol acyltransferase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGCGAAGGCGGCGGTGGCGGCGCTGAACGCAAACCTGGAAGCACACGTG |
| Internal bar code: | TCCATTTTGATGGGGAGTGCCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1528 |
| LEAP-Seq percent confirming: | 60.0 |
| LEAP-Seq n confirming: | 3 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGCAGCTGTCACACATACC |
| Suggested primer 2: | GCTGTGTTGGATTTCGCGTT |