Insertion junction: CLIP2.041451_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.041451
Chromosome:chromosome 9
Location:2162040
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre09.g395600 OBGC,OBGC1 Factor possibly involved in assembly of the ribosomal 50S subunit; (1 of 1) PTHR11702:SF3 - GTP-BINDING PROTEIN 10 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTCCCAGGACATGAAAGGCAAAAGTTTCCGCTTAGCATCTCAATTCCTCT
Internal bar code:GTCCTATTGTTGAGGGTGTCTT

Confirmation - LEAP-Seq

LEAP-Seq distance:2125
LEAP-Seq percent confirming:98.5916
LEAP-Seq n confirming:70
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:71

Suggested primers for confirmation by PCR

Suggested primer 1:GTTGGAGTGCTGCTTGTTGG
Suggested primer 2:GCTGAGGATGACGAGGATGG