| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.041460 |
| Chromosome: | chromosome 12 |
| Location: | 7052570 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g560350 | CNK2 | (1 of 1) PTHR24361//PTHR24361:SF410 - MITOGEN-ACTIVATED KINASE KINASE KINASE // SUBFAMILY NOT NAMED; NIMA-Related Kinase 2 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGGAGGGGAGGGTCTGGCGGCTGATCGCAGGAGCAGCGCCTGAGCGGCC |
| Internal bar code: | TAGTGCGTGCATAGCGGAGTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 254 |
| LEAP-Seq percent confirming: | 87.5 |
| LEAP-Seq n confirming: | 7 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGAAGGTGTTGGGGATGGG |
| Suggested primer 2: | GGGAGAGGTTGGGTCATGTG |