Insertion junction: CLIP2.041481_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.041481
Chromosome:chromosome 14
Location:2554287
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre14.g624900 FAP171 Flagellar Associated Protein 171; (1 of 5) IPR000104//IPR016024 - Antifreeze protein, type I // Armadillo-type fold intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTCGGCGTGACGGCTCGTCATGCGAGGTGGAGGGTTGCTGGCGCATGATA
Internal bar code:TATCAAGGGAAACCGAGATTCT

Confirmation - LEAP-Seq

LEAP-Seq distance:2912
LEAP-Seq percent confirming:98.4615
LEAP-Seq n confirming:64
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:65

Suggested primers for confirmation by PCR

Suggested primer 1:TCATGATCTCGAGTCGCAGC
Suggested primer 2:CGTGTGGCTGCAACATTTGA