| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.041498 |
| Chromosome: | chromosome 12 |
| Location: | 3482449 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g497100 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCGATGGCTGGCCGCACCCAGTCCGGCAGCGTCGGCGCGGGCGGCATAG |
| Internal bar code: | GGTCTCAGGTCCATAGGTCAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1594 |
| LEAP-Seq percent confirming: | 86.6667 |
| LEAP-Seq n confirming: | 26 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 30 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCTCAGCAAGAGCCAGTTC |
| Suggested primer 2: | CTTGGACTCGGTGTTGTCGA |