| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.041501 |
| Chromosome: | chromosome 9 |
| Location: | 5536584 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g409300 | (1 of 5) PF06863 - Protein of unknown function (DUF1254) (DUF1254) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCAACTGGACACTCACCTGGAACTTCACCCGGTGAGCGCCTGTGCGAGCG |
| Internal bar code: | GGTATCGACATTCGTATTTTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1319 |
| LEAP-Seq percent confirming: | 46.1538 |
| LEAP-Seq n confirming: | 18 |
| LEAP-Seq n nonconfirming: | 21 |
| LEAP-Seq n unique pos: | 39 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGGGTCAGGGAAACAGTAA |
| Suggested primer 2: | GTGTTCAATGTGCTGGGCAG |