| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.041507 |
| Chromosome: | chromosome 11 |
| Location: | 4367767 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g482841 | (1 of 2) K11145 - ribonuclease III family protein [EC:3.1.26.-] (K11145) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTGGAGCTGGAGTTAGATATGCTGCCTCAGGGGCAGCAGGCGAGGCCAC |
| Internal bar code: | TTAAGGGCCAGCCAATGTAGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2731 |
| LEAP-Seq percent confirming: | 85.7143 |
| LEAP-Seq n confirming: | 42 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 49 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAGTGCCAGCTTGCATCACA |
| Suggested primer 2: | CGTCAAACTCATAGCGCGTG |