| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.041510 |
| Chromosome: | chromosome 16 |
| Location: | 5569349 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g681700 | NUOS4B | (1 of 2) K03937 - NADH dehydrogenase (ubiquinone) Fe-S protein 4 (NDUFS4); NADH:ubiquinone oxidoreductase subunit | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCAGATAGCGCGCAGCCTTTCCTGCGTTTGCGCCCGTGCCGCTGCCGGAG |
| Internal bar code: | CTCATGTTATTCTAGGAAATGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3046 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 42 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 42 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTACGACCGCCGGATTTTTG |
| Suggested primer 2: | TGTATGCTGCCCTCATTGCA |