| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.041515 |
| Chromosome: | chromosome 14 |
| Location: | 3741470 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g631650 | FAP406 | Flagellar Associated protein 406 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTTGGTGCGAGCGGCAGCTGGAGTGGCGTGGCCGTGAGTGAGCTGCCCG |
| Internal bar code: | TGGCAGTCGATATACAGGAGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1805 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 45 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 45 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGCCAACCTCAGCTCTCAC |
| Suggested primer 2: | GTATGTGCTGCTTGATGCCG |