| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.041516 |
| Chromosome: | chromosome 16 |
| Location: | 7534391 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g683035 | (1 of 2) IPR003593//IPR027417 - AAA+ ATPase domain // P-loop containing nucleoside triphosphate hydrolase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCTGCCGCTGCTGAGGTGCCGAACAAGGTCCTTCGTGCTCCTGCTGCGG |
| Internal bar code: | CACTTAAAAGGGCTACAAAGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2767 |
| LEAP-Seq percent confirming: | 78.481 |
| LEAP-Seq n confirming: | 62 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 79 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTAGATGGCGGTGGTGTTCA |
| Suggested primer 2: | AGAGATACATCGCCAGCACG |