Insertion junction: CLIP2.041519_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.041519
Chromosome:chromosome 1
Location:2211534
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g012400 (1 of 4) PTHR22911//PTHR22911:SF6 - ACYL-MALONYL CONDENSING ENZYME-RELATED // SOLUTE CARRIER FAMILY 35 MEMBER G1 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CACTGCTTTCCCCTGCCACACCGCTTTCTCCCCCTCCCAACCCCCACGGG
Internal bar code:TGAGTACTCTTACGTAGAAGTG

Confirmation - LEAP-Seq

LEAP-Seq distance:134
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:4
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:CACCTGCTTCTACCTGGCAA
Suggested primer 2:GGCAGTTAGGGGTGTGTACC