Insertion junction: CLIP2.041551_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.041551
Chromosome:chromosome 2
Location:256977
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g074758 CYG8 (1 of 70) 4.6.1.2 - Guanylate cyclase / Guanylyl cyclase; Adenylate/guanylate cyclase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTGGTGGCGATGGGTTGCGTGGTCCTGGGGTGCGGCGCGCAGTGGTGGCG
Internal bar code:GGATTCTTCAGTGTCGGTAGAG

Confirmation - LEAP-Seq

LEAP-Seq distance:2618
LEAP-Seq percent confirming:87.5
LEAP-Seq n confirming:14
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:16

Suggested primers for confirmation by PCR

Suggested primer 1:CAAAGCACATGGGGGAAAGC
Suggested primer 2:CCAATCTCTGTGACCTCGGG