| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.041557 |
| Chromosome: | chromosome 12 |
| Location: | 281527 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g801338 | (1 of 2) K08592 - sentrin-specific protease 1 [EC:3.4.22.68] (SENP1) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTGCACCAGCAGGCCACGGTAAAACTCAGCTCGTACGGGACCACATTGT |
| Internal bar code: | TTCCTAGGGTTATTTTGGCGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3326 |
| LEAP-Seq percent confirming: | 62.5 |
| LEAP-Seq n confirming: | 25 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 40 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTCAGCAGCGTCACGTACT |
| Suggested primer 2: | GTGGCATATGTAGGGAGGGC |