| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.041563 |
| Chromosome: | chromosome 2 |
| Location: | 1161343 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g081176 | (1 of 6) IPR001537//IPR029028 - tRNA/rRNA methyltransferase, SpoU type // Alpha/beta knot methyltransferases | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGCGCAGTACATCCTCTACCGTGCGGGCCACTACGACTCTATTGCTGAG |
| Internal bar code: | TGGGTATACTCCATGAATGAAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1285 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 22 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCAACCGTGAACACACTTC |
| Suggested primer 2: | CTTGAGAGCGTGTGGACACT |