| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.041570 |
| Chromosome: | chromosome 1 |
| Location: | 1659745 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g009101 | (1 of 37) PF13639 - Ring finger domain (zf-RING_2) | 3'UTR | |
| Cre01.g009150 | (1 of 1) PF14329 - Domain of unknown function (DUF4386) (DUF4386) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCGACGCATGATGGCGTGTGAGGTCCCAGTCATGTGCACACCTGCACGA |
| Internal bar code: | ACATTTCGCTCTTATGTTAGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2387 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 54 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 54 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAGTGGCGTGAAGAAGCAGG |
| Suggested primer 2: | GTGTGCAAGCCTTGGGAAAG |