| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.041596 |
| Chromosome: | chromosome 16 |
| Location: | 2259542 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g658526 | MNME1,TGD3,MNME | (1 of 1) K03977 - GTP-binding protein (engA); Putative ABC transport system ATP-binding protein | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAGGGAGCCTCTCGTCTCGGCCGCCATGCCTGGCTCCGCGTACAGCGAGG |
| Internal bar code: | TGTTATATGCCGCCCCGTGCGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1501 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 23 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 23 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAATCATAACCCCACCCCA |
| Suggested primer 2: | GAATTGCCATGGCGGATGAC |