Insertion junction: CLIP2.041598_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.041598
Chromosome:chromosome 3
Location:5409168
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g183750 OPR16 OctotricoPeptide Repeat protein 16 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCAGTGTGTAATCTAGATGGGCAGTAGGCGGTGCGCGCCACACTGTCACA
Internal bar code:TTTGAGTCTCCATAAAATCAGG

Confirmation - LEAP-Seq

LEAP-Seq distance:2741
LEAP-Seq percent confirming:88.0
LEAP-Seq n confirming:44
LEAP-Seq n nonconfirming:6
LEAP-Seq n unique pos:50

Suggested primers for confirmation by PCR

Suggested primer 1:CGGGTTCGGCATCGGTAATA
Suggested primer 2:GCTGCTCCTGTCCATATCCC