| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.041600 |
| Chromosome: | chromosome 7 |
| Location: | 5433187 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g350750 | PTO1,PTOX1 | (1 of 2) K17893 - ubiquinol oxidase (AOX1, AOX2); Plastid terminal oxidase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCAATACAGCCGTGACATGGGGGGGAGGTAGCCGGGATGCTTTGGGCTGC |
| Internal bar code: | GCACCTCCTCGAGTTGAATGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1175 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 6 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAACCCTTCTCTCCAGCAT |
| Suggested primer 2: | TAATGCCCTCGACTCCTCCA |