Insertion junction: CLIP2.041604_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.041604
Chromosome:chromosome 16
Location:704970
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g691353 (1 of 14) PF13371 - Tetratricopeptide repeat (TPR_9) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCGTAGGGTCCACGGCCAACAGCTTCTCGAAAGAGGATGAAGCAGCGTCG
Internal bar code:CGAAGGACGTTCGATTAGGTCA

Confirmation - LEAP-Seq

LEAP-Seq distance:1875
LEAP-Seq percent confirming:63.7681
LEAP-Seq n confirming:44
LEAP-Seq n nonconfirming:25
LEAP-Seq n unique pos:69

Suggested primers for confirmation by PCR

Suggested primer 1:CCGCTTTCAAACGCTGAACA
Suggested primer 2:CCTGTGCTCCCCTAGTTTGG