Insertion junction: CLIP2.041620_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.041620
Chromosome:chromosome 12
Location:5512102
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g530300 FKB16C,FKB6,FKB16-3 (1 of 1) PTHR10516//PTHR10516:SF264 - PEPTIDYL-PROLYL CIS-TRANS ISOMERASE // FK506-BINDING PROTEIN 1; peptidyl-prolyl cis-trans isomerase, FKBP-type intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATTTCTTGGATAAAGTTTGGTTTCGACAACAGTGTCCCTTCCCCGGCCAG
Internal bar code:TCGGGTCTGTCAAGCGAGTAGG

Confirmation - LEAP-Seq

LEAP-Seq distance:3325
LEAP-Seq percent confirming:88.5714
LEAP-Seq n confirming:31
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:35

Suggested primers for confirmation by PCR

Suggested primer 1:AGGTGGTGGTGCACTATGTG
Suggested primer 2:ATACTAGCCCCTCAGCCCAA