| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.041634 |
| Chromosome: | chromosome 12 |
| Location: | 2809023 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g503650 | SMM40 | S-adenosyl-L-methionine-dependent methyltransferase; (1 of 1) PTHR10108//PTHR10108:SF894 - METHYLTRANSFERASE // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTTGGGGGGAAGGATATGGGAGGATGCGGAGGGAGGCCACAGGCGCGAC |
| Internal bar code: | GGACACAGTGTAAGTGCGGTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2329 |
| LEAP-Seq percent confirming: | 92.2581 |
| LEAP-Seq n confirming: | 143 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 155 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGCTAATGGACCCAAGGTG |
| Suggested primer 2: | AGGACAAAGGCACAACCACA |