| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.041642 |
| Chromosome: | chromosome 12 |
| Location: | 4722783 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g522800 | (1 of 781) IPR000104 - Antifreeze protein, type I | 3'UTR | |
| Cre12.g522850 | HEL55 | (1 of 1) K12835 - ATP-dependent RNA helicase DDX42 (DDX42, SF3B125); DEAD/DEAH box helicase similar to Eukaryotic Initiation Factor 4A | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGAGGCCCCGCTACCACCGCCACCCCCGTACGACGATGACGATGAGCCCC |
| Internal bar code: | GAGTGGTGAAACGAGTGTAGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 346 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 8 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGACCTTCGTGCAGCTACC |
| Suggested primer 2: | GCGCTCCCATGTCCTACTAC |